cd25 microbead kit Search Results


cd25 microbead kit  (Miltenyi Biotec)
95
Miltenyi Biotec cd25 microbead kit
A , <t>CD25-depleted</t> T cells from Nur77 GFP mice were stimulated for three days with anti-CD3/CD28 mAbs, followed by a seven day rest with IL-2. After three rounds of stimulation, cells were stained for PD-1 and Tim-3 and analyzed by flow cytometry. Representative of 5 technical replicates per experiment, repeated with n=5 mice. B , WT C57BL/6 mice were infected with LCMV Arm. After 30d, spleens were harvested for flow cytometry (n=5 mice, mean ± SD). representative of three independent experiments C , Tim-3 + vs. Tim-3 − CD8 + cells were further analyzed for expression of activation and differentiation markers shown in the histograms. D , splenocytes from the same experiments as panels b-c were stained with LCMV tetramers plus α Tim-3. (n=5 mice, mean ± SD) representative of three independent experiments. **p<0.01 by two-tailed paired Student’s t test. E , C57Bl/6 mice previously infected with LCMV-Arm (>30d.p.i.) were challenged with LM-GP33 and Tet − vs. Tet + CD8 + splenocytes were analyzed for KLRG1 and Tim-3 four days post-challenge. Representative of three independent experiments (n=5).
Cd25 Microbead Kit, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/cd25 microbead kit/product/Miltenyi Biotec
Average 95 stars, based on 1 article reviews
cd25 microbead kit - by Bioz Stars, 2026-02
95/100 stars
  Buy from Supplier
cd25 microbeads ii, human  (Miltenyi Biotec)
97
Miltenyi Biotec cd25 microbeads ii, human
A , <t>CD25-depleted</t> T cells from Nur77 GFP mice were stimulated for three days with anti-CD3/CD28 mAbs, followed by a seven day rest with IL-2. After three rounds of stimulation, cells were stained for PD-1 and Tim-3 and analyzed by flow cytometry. Representative of 5 technical replicates per experiment, repeated with n=5 mice. B , WT C57BL/6 mice were infected with LCMV Arm. After 30d, spleens were harvested for flow cytometry (n=5 mice, mean ± SD). representative of three independent experiments C , Tim-3 + vs. Tim-3 − CD8 + cells were further analyzed for expression of activation and differentiation markers shown in the histograms. D , splenocytes from the same experiments as panels b-c were stained with LCMV tetramers plus α Tim-3. (n=5 mice, mean ± SD) representative of three independent experiments. **p<0.01 by two-tailed paired Student’s t test. E , C57Bl/6 mice previously infected with LCMV-Arm (>30d.p.i.) were challenged with LM-GP33 and Tet − vs. Tet + CD8 + splenocytes were analyzed for KLRG1 and Tim-3 four days post-challenge. Representative of three independent experiments (n=5).
Cd25 Microbeads Ii, Human, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/cd25 microbeads ii, human/product/Miltenyi Biotec
Average 97 stars, based on 1 article reviews
cd25 microbeads ii, human - by Bioz Stars, 2026-02
97/100 stars
  Buy from Supplier

Image Search Results


A , CD25-depleted T cells from Nur77 GFP mice were stimulated for three days with anti-CD3/CD28 mAbs, followed by a seven day rest with IL-2. After three rounds of stimulation, cells were stained for PD-1 and Tim-3 and analyzed by flow cytometry. Representative of 5 technical replicates per experiment, repeated with n=5 mice. B , WT C57BL/6 mice were infected with LCMV Arm. After 30d, spleens were harvested for flow cytometry (n=5 mice, mean ± SD). representative of three independent experiments C , Tim-3 + vs. Tim-3 − CD8 + cells were further analyzed for expression of activation and differentiation markers shown in the histograms. D , splenocytes from the same experiments as panels b-c were stained with LCMV tetramers plus α Tim-3. (n=5 mice, mean ± SD) representative of three independent experiments. **p<0.01 by two-tailed paired Student’s t test. E , C57Bl/6 mice previously infected with LCMV-Arm (>30d.p.i.) were challenged with LM-GP33 and Tet − vs. Tet + CD8 + splenocytes were analyzed for KLRG1 and Tim-3 four days post-challenge. Representative of three independent experiments (n=5).

Journal: bioRxiv

Article Title: Tim-3 co-stimulation promotes short-term effector T cells, restricts memory precursors and is dispensable for T cell exhaustion

doi: 10.1101/179002

Figure Lengend Snippet: A , CD25-depleted T cells from Nur77 GFP mice were stimulated for three days with anti-CD3/CD28 mAbs, followed by a seven day rest with IL-2. After three rounds of stimulation, cells were stained for PD-1 and Tim-3 and analyzed by flow cytometry. Representative of 5 technical replicates per experiment, repeated with n=5 mice. B , WT C57BL/6 mice were infected with LCMV Arm. After 30d, spleens were harvested for flow cytometry (n=5 mice, mean ± SD). representative of three independent experiments C , Tim-3 + vs. Tim-3 − CD8 + cells were further analyzed for expression of activation and differentiation markers shown in the histograms. D , splenocytes from the same experiments as panels b-c were stained with LCMV tetramers plus α Tim-3. (n=5 mice, mean ± SD) representative of three independent experiments. **p<0.01 by two-tailed paired Student’s t test. E , C57Bl/6 mice previously infected with LCMV-Arm (>30d.p.i.) were challenged with LM-GP33 and Tet − vs. Tet + CD8 + splenocytes were analyzed for KLRG1 and Tim-3 four days post-challenge. Representative of three independent experiments (n=5).

Article Snippet: CD25 depletion was done using a CD25 microbead kit (Miltenyi).

Techniques: Staining, Flow Cytometry, Infection, Expressing, Activation Assay, Two Tailed Test